Column:

GP-C18, 5 µm, 120 Å, 4.6 × 150 mm

Mobile phase:

A: ACN : 0.1M NaAc (pH 6.5) = 7 : 93 (v/v);
B: ACN : H₂O = 4 : 1 (v/v)

    Time (min):
               B (%):

0   15   25   30   30.01  40  40.01
0    0    40   40   100   100  0

Flow rate:

1.0 mL/min

Temperature:

36 ^oC

Detection:

UV 254 nm

Injection Volume:

4 µL

Sample:

Brain protein hydrolysate （脑蛋白水解物）